Force Left Primer Start||
Force Right Primer Start||
Force Left Primer End||
Force Right Primer End||
General Primer Picking Conditions
Upload the settings from a file
Objective Function Penalty Weights for Primers
Objective Function Penalty Weights for Primer Pairs
Internal Oligo (Hyb Oligo) General Conditions
Objective Function Penalty Weights for Internal Oligos (Hyb Oligos)
Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010,2011,2012
Whitehead Institute for Biomedical Research, Steve Rozen, Andreas Untergasser and Helen Skaletsky.
All rights reserved.
This file is part of the primer3web suite.
The primer3 suite and libraries are free software;
you can redistribute them and/or modify them under the terms
of the GNU General Public License as published by the Free
Software Foundation; either version 2 of the License, or (at
your option) any later version.
This software is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.
You should have received a copy of the GNU General Public License
along with this software (file gpl-2.0.txt in the source
distribution); if not, write to the Free Software
Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA
THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS
"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR
A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT
OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT
LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE,
DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE
OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
We request that use of this software be cited in
and Helen J. Skaletsky (2000)
Primer3 on the WWW for general users and for biologist programmers.
In: Krawetz S, Misener S (eds)
Bioinformatics Methods and Protocols: Methods in Molecular Biology.
Humana Press, Totowa, NJ, pp 365-386
Source code available at
Initial development of Primer3 was funded by Howard Hughes Medical
Institute and by the National Institutes of Health, National Human
Genome Research Institute under grants R01-HG00257 (to David C. Page)
and P50-HG00098 (to Eric S. Lander)
but ongoing development and maintenance are not currently funded.
Primer3 was a complete re-implementation
of an earlier program:
Primer 0.5 (Steve Lincoln, Mark Daly, and Eric
(input.htm v 3.0.0)